The application also provides tools for optimizing image display and analyzing images or kinetic data. The visual output represents the number of photons emittedcm 2 as a pseudocolor image where the maximum is red and minimum is purple. To do this, an in vitro measurement of a stably integrated cell line of interest must be taken using the camera. Perkinelmer caliper ivis spectrum hardware manual pdf download. B activation in vitro, however, techniques involving the imaging of nf. Sign on log book once you entered the animal imaging room. In vivo imaging systems ivis detection of a neuroinvasive. Ivis spectrum in vivo imaging system university imaging. Ivis and living image are registered trademarks or trademarks of caliper life sciences, inc.
For instructions on using the system software, please see the living image software users manual. The lower panels of a show xenogen system images of the identical plate of cells shown on the upper panels of a. To start living image software under windows, look for the living image soft ware icon in the startprogramsliving image. If you have questions regarding this manual or the ivis imaging system, please. Imaging results living image software with high resolution filters. Xenogen ivis 100 in vivo imaging system standard operation. Sopguidelines for optical imaging using iviskinetic. The planes in the 3 side panels below can be used to approximate to. Customize the ivis lumina ii with your own filter combinations.
A windowsbased computer system for data acquisition and analysis. After 10 min following the injection of luciferin, image the mice through the living image software. System manual ivis lumina xr realtime bioluminescence. Breluc oslo mice from xenogen corporation alameda, california provide a great model for studying and imaging anticancer drugs that target nf. The living image software for automated image acquisition, postprocessing, and data analysis. For more details on the setup and manual control of the infusion pump, see the ivis syringe injection. Room cle c 301 is where you can find ivif staff and 3 powerful workstations for analyses on dedicated software imaris cell packeage, living image 3. This software was designed and made by very same person who made living image software almost two decade ago. For quantification of the bioluminescence signal using roi, it is advisable to use the units photons and not counts see the user manual for the living image software from xenogen for more. Living image advanced in vivo imaging software designed for the ivis lumina platform simplifies even the most complex image acquisition and analysis of 2d bioluminescent and fluorescent probes. Please refer to the living image software manual for instructions on setting binning, exposure time, and fstop values. Perkinelmer has led the way for over 25 years with powerful software for its in. For instructions on using the syst em software, please see the living image software users manual. Log on to the computer and start the living image 2.
An optimized set of high efficiency filters and spectral unmixing algorithms lets you take full advantage of bioluminescent and fluorescent reporters across the blue to near infrared wavelength region. With streamlined interfaces and workflows for longitudinal analysis of 2d data including coregistration with xray in applicable ivis lumina systems. Image math tool spectral unmixing advanced imaging tips. An optimized set of high efficiency filters and spectral unmixing algorithms lets you take full advantage of bioluminescent and fluorescent reporters across the blue to. A represents the image taken with the primary excitation filter.
Each file in an image folder is required to reconstruct the data set in the living image software. Manual binning in the living image toolspreferencecamera settings box. When manual is selected, use the tab button to cycle through the 3 tool sets. Xenogen, discovery in the living organism, bioware, ivis, living image and lpta are trademarks andor trade names of xenogen corporation. Xgi8 gas anesthesia system contact information please call 1. Jun 27, 2006 for quantification of the bioluminescence signal using roi, it is advisable to use the units photons and not counts see the user manual for the living image software from xenogen for more. Advanced acquisition and analysis tools for ivis lumina imaging systems.
Manual coregistration provides panning, shrinkgrow and rotational position options to overlay the organ atlas to the surface shape. Xenogen corporation xenogen corporation, living image software manual version 2. Sopguidelines for optical imaging using iviskinetic system. This living image software manual is organized according to thepanels and menu items shown in figure 1. Click the export graphics button you can save it as tiff format. Xenogen ivis 100 in vivo imaging system standard operation protocol basic operation for acquiring image 1. Analysis software can be purchased from caliper lifesciences to allow analysis by users on their own computers. Optical imaging utilizes a variety of labeled molecules. The xenogen ivis system combines the ability to image in vitro or in vivo, fluorescence or bioluminescence, and 2d or 3d. Allison poussard 1, michael patterson 1, katherine taylor 1, alexey seregin 1.
In vivo imaging systems ivis detection of a neuroinvasive encephalitic virus. Living image software creates a custom environment that is used for both data acquisition and analysis as described in this manual. The ivis spectrum is the most versatile and advanced in vivo imaging system available on the market today. Xenogen ivis 100 in vivo imaging system university. Its capable of imaging bioluminescence and fluorescence. Which imaging system do you recommend for small animal in. Braf inhibition increases tumor infiltration by t cells and. Towards a framework for analysis of biophotonic images of mouse models of cancer. Luciferin and in vivo imaging university of chicago. It will take several minutes for the check up of the system and for the camera to cool down to 90 0c. Living image software xenogen was used to analyze data. Xenogen ivis 200 comparatively inexpensive technique for noninvasive study of molecular targets inside the body of the living animals.
The software is easy to use, the detectors are excellent and the base is nice and stable. You can check the current temperature by clicking on the red bar. Living image software and igor pro run on both macintosh and windows computers. Custom designed for in vivo bioluminescent imaging settings are analogous to photography software images are acquired in a two step process sensitivity is controlled by integration time, fstop, and binning living image controls ivis and provides image analysis tools fluorescence. Xenogen ivis 100 in vivo imaging system university imaging. Currently, there are various assays available to study nf. Select abk subtraction mode from the results dropdown list. Xenogen ivis200 central microscopy research facility. The xenogen ivis200 system is a highsensitivity, low noise, in vivo imaging technology platform that allows noninvasive visualization and tracking of cellular and genetic activity within a living organism, in real time. This user manual explains how to acquire optical and volumetric image data on the ivis spectrum and analyze the data using the living image software. Ivis fluorescent imaging guide university of chicago. Following initial imaging with an open filter, initiate a dlit 3dimensional imaging using the sequence analysis and image wizard setup for bioluminescent firefly luciferase. In vivo bioluminescent imaging of mammary tumors using. Caliper xenogen ivis lumina with the livingimage software hopkinton, ma.
Oxidative injury is a common consequence of bmpr2 mutations. Analysis of imaging data is carried out on two pc workstations with living image analysis standalone software, or the software can be purchased from xenogen caliper life sciences to allow analysis by users on their own computers. Realtime in vivo imaging of transgenic bioluminescent. For instructions on how to acquire fluorescent images using the xfo12 fluorescence equipment, please see chapter 9, page 33. The ivis 100 imaging system is a highly sensitive and large field of view imaging system that offers users the flexibility to image bioluminescent reporters both in vivo and in vitro. Results detection of orangered, farred, and gfp expression in tissue culture cells with the xenogen system. The lens provides high light collection at f1, an adjustable field of view from 423 cm, uniform light collection, and superior resolution with single cell sensitivity for in vitro or ex vivo use. Commands and controlsassociated with the system control panel, the image display.
High throughput quantitative bioluminescence imaging for. Developing an animal model of dupuytrens disease by. Analysis of imaging data is available to all users. With streamlined interfaces and workflows for longitudinal analysis of 2d data including coregistration with xray in. Perkin elmer caliper ivis spectrum in vivo system cell.
Expression of therapeutic proteins after delivery of. This guide will walk you through the steps of manually entering your sequences for the spectral unmixing procedure. Check to make sure the correct image number is highlighted for a. Image processing after the image is successfully acquired and an image window and tool palette should appear. Towards a framework for analysis of biophotonic images of. Which imaging system do you recommend for small animal invivo imaging. Falsecolor overlays represent the spectrum of photon radiance or flux i. Computer is kept always on, no need to turn on computer 3. B represent the image taken with the background excitation filter. Image data consists of folders, not individual files. Noninvasive optical tracking of red fluorescent protein. Xenogen corporation 860 atlantic avenue alameda, california 94501 usa phone 510 2916100.
Gene expression array analysis affymetrix cel files were loaded into dchip array analysis software. For questions on how to use these two features please see the respective quick reference guide associated with. Xenogens ivis 200 series and living image software 1 in vivo imaging xenogens ivis 200 series and living image software 2 what will be covered. Concept tech note 6 planar spectral imaging 1 of 7 the unique spectral signatures of the luciferase em ission spectrum and the optical properties of tissue enable the living image software to determine the depth and intensity of light sources inside a living animal. Three weeks following the expansion of fluorescent cells, fluorescenceactivated cell sorting facs was performed at the johns hopkins. The xenogen xgi8 gas anesthesia system is designed to work with the ivis imaging system, a technology from xenogen that allows researchers to use realtime in vivo imaging to monitor and record cellular and genetic.
You can control the infusion pump in the living image software or manually. Fluorescent choose this option to acquire a fluorescent image. Concept tech note 7 ivis syringe injection system 1 of 3 the ivis syringe injection system is designed for use with the ivis kinetic imaging system. Same as above but the user must first activate show igortips from the top level help menu. For more details on the setup and manual control of the infusion pump, see the ivis syringe injection system instructions from caliper or the phd 222000 syringe. Living image software simplifies even the most advanced optical imaging protocol to facilitate success the first time and every time. In vivo bioluminescent imaging of mammary tumors using ivis.
The software s new design creates an intuitive, seamless workflow to streamline acquisition through analysis. Messenger rna has received little attention as a potential therapeutic agent. Once the coregistration is set, organs may be removed or selected to appear in the 3d image along with the. These settings can be changed at any time during an experiment without impacting the quantitative result. In living image software, exposure time, fstop and pixel binning can be optimized based on the expression level of the cell line. Software images are acquired in a two step process sensitivity is controlled by integration time, fstop, and binning living image controls ivis and provides image analysis tools fluorescence tissue autofluorescence and instrument background can be subtracted. Ivis spectrum in vivo imaging system university imaging centers. Living image software provides powerful new features such as wizard g. Bioluminescence imaging bli has emerged during the past five years as the preeminent method for. Once compiled, images were quantified for detection of fluorescent signal emitted from the forepaw using xenogen living image software. Realtime in vivo imaging of transgenic bioluminescent blood. Braf inhibition increases tumor infiltration by t cells. Xenogen corporation, 860 atlantic avenue, alameda, ca, 94501, usa toll free 877.